The weak CD8+ CTL response to an influenza hemagglutinin epitope reflects limited T cell availability.
Identifieur interne : 001B99 ( Main/Exploration ); précédent : 001B98; suivant : 001C00The weak CD8+ CTL response to an influenza hemagglutinin epitope reflects limited T cell availability.
Auteurs : W. Cao [États-Unis] ; B A Myers-Powell ; T J BracialeSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1996.
Descripteurs français
- KwdFr :
- Animaux, Cellules cancéreuses en culture, Données de séquences moléculaires, Famille multigénique (immunologie), Hémagglutinines virales (immunologie), Lymphocytes T cytotoxiques (immunologie), Numération des lymphocytes, Présentation d'antigène, Récepteurs aux antigènes des cellules T (génétique), Souris, Souris de lignée BALB C, Sous-populations de lymphocytes T (), Sous-populations de lymphocytes T (immunologie), Séquence d'acides aminés, Virus de la grippe A (immunologie), Épitopes (immunologie).
- MESH :
- génétique : Récepteurs aux antigènes des cellules T.
- immunologie : Famille multigénique, Hémagglutinines virales, Lymphocytes T cytotoxiques, Sous-populations de lymphocytes T, Virus de la grippe A, Épitopes.
- Animaux, Cellules cancéreuses en culture, Données de séquences moléculaires, Numération des lymphocytes, Présentation d'antigène, Souris, Souris de lignée BALB C, Sous-populations de lymphocytes T, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antigen Presentation, Epitopes (immunology), Hemagglutinins, Viral (immunology), Influenza A virus (immunology), Lymphocyte Count, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Multigene Family (immunology), Receptors, Antigen, T-Cell (genetics), T-Lymphocyte Subsets (classification), T-Lymphocyte Subsets (immunology), T-Lymphocytes, Cytotoxic (immunology), Tumor Cells, Cultured.
- MESH :
- chemical , genetics : Receptors, Antigen, T-Cell.
- chemical , immunology : Epitopes, Hemagglutinins, Viral.
- classification : T-Lymphocyte Subsets.
- immunology : Influenza A virus, Multigene Family, T-Lymphocyte Subsets, T-Lymphocytes, Cytotoxic.
- Amino Acid Sequence, Animals, Antigen Presentation, Lymphocyte Count, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Tumor Cells, Cultured.
Abstract
One of the two class I MHC (H-2Kd)-restricted immunogenic regions identified on the influenza virus strain A/Japan/57 (H2N2) hemagglutinin (HA) encompasses two distinct, partially overlapping epitopes. These epitopes map to residues 204 to 212 (JHA 204-212) and 210 to 219 (JHA 210-219), respectively. When we investigated the magnitude of the CTL responses of H-2d BALB/c mice to these two epitopes, we found that the JHA 204-212 nonamer epitope is immunodominant, eliciting vigorous CTL responses in BALB/c mice immunized with A/Japan/57 virus. In contrast, the CTL response to the JHA 210-219 decamer epitope was weak and variable. The subdominance of the JHA 210-219 was not due to low affinity binding of JHA 210-219 to H-2Kd or to inefficient processing of this epitope in vivo. Rather, an analysis of CTL precursor (pCTL) frequency by limiting dilution showed that the frequency of pCTL to the JHA 210-219 epitope was at least 10-fold lower than the frequency of pCTL to the JHA 204-212 epitope, implying that the low and variable response to the JHA 210-219 epitope was due to a limited number of CD8+ T cell precursors directed to JHA 210-219. This hypothesis was further supported by the finding of limited heterogeneity in reactivity pattern displayed by short term bulk cultures of the JHA 210-219-specific CTLs for cross-reactive epitopes. Implications of these findings for vaccine design and for T lymphocyte function and repertoire development are discussed.
PubMed: 8752895
Affiliations:
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Carter Center for Immunology Research, University of Virginia Health Sciences Center, Charlottesville 22908</wicri:regionArea><wicri:noRegion>Charlottesville 22908</wicri:noRegion></affiliation></author><author><name sortKey="Myers Powell, B A" sort="Myers Powell, B A" uniqKey="Myers Powell B" first="B A" last="Myers-Powell">B A Myers-Powell</name></author><author><name sortKey="Braciale, T J" sort="Braciale, T J" uniqKey="Braciale T" first="T J" last="Braciale">T J Braciale</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8752895</idno><idno type="pmid">8752895</idno><idno type="wicri:Area/PubMed/Corpus">000397</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000397</idno><idno type="wicri:Area/PubMed/Curation">000397</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000397</idno><idno type="wicri:Area/PubMed/Checkpoint">000359</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000359</idno><idno type="wicri:Area/Ncbi/Merge">001329</idno><idno type="wicri:Area/Ncbi/Curation">001329</idno><idno type="wicri:Area/Ncbi/Checkpoint">001329</idno><idno type="wicri:doubleKey">0022-1767:1996:Cao W:the:weak:cd</idno><idno type="wicri:Area/Main/Merge">001C64</idno><idno type="wicri:Area/Main/Curation">001B99</idno><idno type="wicri:Area/Main/Exploration">001B99</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The weak CD8+ CTL response to an influenza hemagglutinin epitope reflects limited T cell availability.</title><author><name sortKey="Cao, W" sort="Cao, W" uniqKey="Cao W" first="W" last="Cao">W. Cao</name><affiliation wicri:level="1"><nlm:affiliation>Beirne B. Carter Center for Immunology Research, University of Virginia Health Sciences Center, Charlottesville 22908, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Beirne B. Carter Center for Immunology Research, University of Virginia Health Sciences Center, Charlottesville 22908</wicri:regionArea><wicri:noRegion>Charlottesville 22908</wicri:noRegion></affiliation></author><author><name sortKey="Myers Powell, B A" sort="Myers Powell, B A" uniqKey="Myers Powell B" first="B A" last="Myers-Powell">B A Myers-Powell</name></author><author><name sortKey="Braciale, T J" sort="Braciale, T J" uniqKey="Braciale T" first="T J" last="Braciale">T J Braciale</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antigen Presentation</term><term>Epitopes (immunology)</term><term>Hemagglutinins, Viral (immunology)</term><term>Influenza A virus (immunology)</term><term>Lymphocyte Count</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Multigene Family (immunology)</term><term>Receptors, Antigen, T-Cell (genetics)</term><term>T-Lymphocyte Subsets (classification)</term><term>T-Lymphocyte Subsets (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Données de séquences moléculaires</term><term>Famille multigénique (immunologie)</term><term>Hémagglutinines virales (immunologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Numération des lymphocytes</term><term>Présentation d'antigène</term><term>Récepteurs aux antigènes des cellules T (génétique)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sous-populations de lymphocytes T ()</term><term>Sous-populations de lymphocytes T (immunologie)</term><term>Séquence d'acides aminés</term><term>Virus de la grippe A (immunologie)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, Antigen, T-Cell</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>T-Lymphocyte Subsets</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Récepteurs aux antigènes des cellules T</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Famille multigénique</term><term>Hémagglutinines virales</term><term>Lymphocytes T cytotoxiques</term><term>Sous-populations de lymphocytes T</term><term>Virus de la grippe A</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>Multigene Family</term><term>T-Lymphocyte Subsets</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antigen Presentation</term><term>Lymphocyte Count</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Données de séquences moléculaires</term><term>Numération des lymphocytes</term><term>Présentation d'antigène</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sous-populations de lymphocytes T</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">One of the two class I MHC (H-2Kd)-restricted immunogenic regions identified on the influenza virus strain A/Japan/57 (H2N2) hemagglutinin (HA) encompasses two distinct, partially overlapping epitopes. These epitopes map to residues 204 to 212 (JHA 204-212) and 210 to 219 (JHA 210-219), respectively. When we investigated the magnitude of the CTL responses of H-2d BALB/c mice to these two epitopes, we found that the JHA 204-212 nonamer epitope is immunodominant, eliciting vigorous CTL responses in BALB/c mice immunized with A/Japan/57 virus. In contrast, the CTL response to the JHA 210-219 decamer epitope was weak and variable. The subdominance of the JHA 210-219 was not due to low affinity binding of JHA 210-219 to H-2Kd or to inefficient processing of this epitope in vivo. Rather, an analysis of CTL precursor (pCTL) frequency by limiting dilution showed that the frequency of pCTL to the JHA 210-219 epitope was at least 10-fold lower than the frequency of pCTL to the JHA 204-212 epitope, implying that the low and variable response to the JHA 210-219 epitope was due to a limited number of CD8+ T cell precursors directed to JHA 210-219. This hypothesis was further supported by the finding of limited heterogeneity in reactivity pattern displayed by short term bulk cultures of the JHA 210-219-specific CTLs for cross-reactive epitopes. Implications of these findings for vaccine design and for T lymphocyte function and repertoire development are discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Braciale, T J" sort="Braciale, T J" uniqKey="Braciale T" first="T J" last="Braciale">T J Braciale</name><name sortKey="Myers Powell, B A" sort="Myers Powell, B A" uniqKey="Myers Powell B" first="B A" last="Myers-Powell">B A Myers-Powell</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Cao, W" sort="Cao, W" uniqKey="Cao W" first="W" last="Cao">W. Cao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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